Virus:Article Title: Dopamine Inputs from the Ventral Tegmental Area into the Medial Prefrontal Cortex Modulate Neuropathic Pain-Associated Behaviors in Mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal c-Fos antibody Abcam Cat#ab190289, RRID:AB_2737414 CaMKII alpha Monoclonal Antibody (6G9) Thermo Fisher Scientific Cat#MA1-048, RRID:AB_325403 Goat Anti-Rabbit IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor 546 Molecular Probes (Invitrogen) Cat#A-11035, RRID:AB_143051 Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 633 Molecular Probes (Invitrogen) Cat#A-21050, RRID:AB_141431 Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 546 Molecular Probes (Invitrogen) CatA-11003, RRID:AB_141370 Bacterial and Virus Strains AAV9.EF1a.DIO.hChR2(H134R)eYFP.WPRE.hGH Addgene (Karl Deisseroth Lab: Creactivated AAV expression plasmids Unpublished) RRID:Addgene_20298 AAV9.EF1a.DIO.eYFP.WPRE.hGH Addgene (Karl Deisseroth Lab: Double Floxed Inverted ORF Control Unpublished) RRID:Addgene_27056 AAVrg-syn-jGCaMP7s-WPRE Addgene RRID:Addgene_104487 AAV9-Ef1a-DIO C1V1 (t/t)-TS-EYFP Addgene RRID:Addgene_35497 Chemicals SCH 23390 hydrochloride Tocris N/A Cat. No. 0925 (S)-(-)-Sulpiride Tocris N/A Cat. No. 0895 Experimental Models: Organisms/Strains B6.SJL-Slc6a3tm1.1(cre)Bkmn/J Mus musculus Jax RRID:IMSR_JAX:006660 B6;129S-Gt(ROSA) 26Sortm32(CAG-COP4*H134R/EYFP)Hze/J Mus musculus Jax RRID:IMSR_JAX:012569 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus Jax RRID:IMSR_JAX:007909 B6.SJL-Slc6a3tm1.1(cre)Bkmn/J x B6;129S-Gt (ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J Mus musculus University of Calgary animal facility (Origin of breeding pairs: Jax) N/A B6.SJL-Slc6a3tm1.1(cre)Bkmn/J x B6.CgGt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus University of Calgary animal facility (Origin of breeding pairs: Jax) N/A Software and Algorithms OriginPro 9.1 Origin RRID:SCR_014212 MATLAB (R2018a) MATLAB RRID:SCR_001622 GraphPad GraphPad RRID:SCR_000306 pClamp Molecular Devices RRID:SCR_011323 TarHeel CV Software ESA Biosciences N/A High Definition Cyclic Voltammetry Suite University of North Carolina N/A Doric Neuroscience Studio Software Doric Lenses N/A Power analysis tool UBC- Rollin Brant https://www.stat.ubc.ca/ rollin/stats/ ssize/n2.html MATLAB custom code Custom code https://github.com/leomol/FPA (Continued on next page) e1 Cell Reports 31, 107812, June 23, 2020 ..
Bioprocessing:Article Title: Dopamine Inputs from the Ventral Tegmental Area into the Medial Prefrontal Cortex Modulate Neuropathic Pain-Associated Behaviors in Mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal c-Fos antibody Abcam Cat#ab190289, RRID:AB_2737414 CaMKII alpha Monoclonal Antibody (6G9) Thermo Fisher Scientific Cat#MA1-048, RRID:AB_325403 Goat Anti-Rabbit IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor 546 Molecular Probes (Invitrogen) Cat#A-11035, RRID:AB_143051 Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 633 Molecular Probes (Invitrogen) Cat#A-21050, RRID:AB_141431 Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 546 Molecular Probes (Invitrogen) CatA-11003, RRID:AB_141370 Bacterial and Virus Strains AAV9.EF1a.DIO.hChR2(H134R)eYFP.WPRE.hGH Addgene (Karl Deisseroth Lab: Creactivated AAV expression plasmids Unpublished) RRID:Addgene_20298 AAV9.EF1a.DIO.eYFP.WPRE.hGH Addgene (Karl Deisseroth Lab: Double Floxed Inverted ORF Control Unpublished) RRID:Addgene_27056 AAVrg-syn-jGCaMP7s-WPRE Addgene RRID:Addgene_104487 AAV9-Ef1a-DIO C1V1 (t/t)-TS-EYFP Addgene RRID:Addgene_35497 Chemicals SCH 23390 hydrochloride Tocris N/A Cat. No. 0925 (S)-(-)-Sulpiride Tocris N/A Cat. No. 0895 Experimental Models: Organisms/Strains B6.SJL-Slc6a3tm1.1(cre)Bkmn/J Mus musculus Jax RRID:IMSR_JAX:006660 B6;129S-Gt(ROSA) 26Sortm32(CAG-COP4*H134R/EYFP)Hze/J Mus musculus Jax RRID:IMSR_JAX:012569 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus Jax RRID:IMSR_JAX:007909 B6.SJL-Slc6a3tm1.1(cre)Bkmn/J x B6;129S-Gt (ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J Mus musculus University of Calgary animal facility (Origin of breeding pairs: Jax) N/A B6.SJL-Slc6a3tm1.1(cre)Bkmn/J x B6.CgGt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus University of Calgary animal facility (Origin of breeding pairs: Jax) N/A Software and Algorithms OriginPro 9.1 Origin RRID:SCR_014212 MATLAB (R2018a) MATLAB RRID:SCR_001622 GraphPad GraphPad RRID:SCR_000306 pClamp Molecular Devices RRID:SCR_011323 TarHeel CV Software ESA Biosciences N/A High Definition Cyclic Voltammetry Suite University of North Carolina N/A Doric Neuroscience Studio Software Doric Lenses N/A Power analysis tool UBC- Rollin Brant https://www.stat.ubc.ca/ rollin/stats/ ssize/n2.html MATLAB custom code Custom code https://github.com/leomol/FPA (Continued on next page) e1 Cell Reports 31, 107812, June 23, 2020 ..
Expressing:Article Title: Dopamine Inputs from the Ventral Tegmental Area into the Medial Prefrontal Cortex Modulate Neuropathic Pain-Associated Behaviors in Mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal c-Fos antibody Abcam Cat#ab190289, RRID:AB_2737414 CaMKII alpha Monoclonal Antibody (6G9) Thermo Fisher Scientific Cat#MA1-048, RRID:AB_325403 Goat Anti-Rabbit IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor 546 Molecular Probes (Invitrogen) Cat#A-11035, RRID:AB_143051 Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 633 Molecular Probes (Invitrogen) Cat#A-21050, RRID:AB_141431 Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 546 Molecular Probes (Invitrogen) CatA-11003, RRID:AB_141370 Bacterial and Virus Strains AAV9.EF1a.DIO.hChR2(H134R)eYFP.WPRE.hGH Addgene (Karl Deisseroth Lab: Creactivated AAV expression plasmids Unpublished) RRID:Addgene_20298 AAV9.EF1a.DIO.eYFP.WPRE.hGH Addgene (Karl Deisseroth Lab: Double Floxed Inverted ORF Control Unpublished) RRID:Addgene_27056 AAVrg-syn-jGCaMP7s-WPRE Addgene RRID:Addgene_104487 AAV9-Ef1a-DIO C1V1 (t/t)-TS-EYFP Addgene RRID:Addgene_35497 Chemicals SCH 23390 hydrochloride Tocris N/A Cat. No. 0925 (S)-(-)-Sulpiride Tocris N/A Cat. No. 0895 Experimental Models: Organisms/Strains B6.SJL-Slc6a3tm1.1(cre)Bkmn/J Mus musculus Jax RRID:IMSR_JAX:006660 B6;129S-Gt(ROSA) 26Sortm32(CAG-COP4*H134R/EYFP)Hze/J Mus musculus Jax RRID:IMSR_JAX:012569 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus Jax RRID:IMSR_JAX:007909 B6.SJL-Slc6a3tm1.1(cre)Bkmn/J x B6;129S-Gt (ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J Mus musculus University of Calgary animal facility (Origin of breeding pairs: Jax) N/A B6.SJL-Slc6a3tm1.1(cre)Bkmn/J x B6.CgGt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus University of Calgary animal facility (Origin of breeding pairs: Jax) N/A Software and Algorithms OriginPro 9.1 Origin RRID:SCR_014212 MATLAB (R2018a) MATLAB RRID:SCR_001622 GraphPad GraphPad RRID:SCR_000306 pClamp Molecular Devices RRID:SCR_011323 TarHeel CV Software ESA Biosciences N/A High Definition Cyclic Voltammetry Suite University of North Carolina N/A Doric Neuroscience Studio Software Doric Lenses N/A Power analysis tool UBC- Rollin Brant https://www.stat.ubc.ca/ rollin/stats/ ssize/n2.html MATLAB custom code Custom code https://github.com/leomol/FPA (Continued on next page) e1 Cell Reports 31, 107812, June 23, 2020 ..
Control:Article Title: Dopamine Inputs from the Ventral Tegmental Area into the Medial Prefrontal Cortex Modulate Neuropathic Pain-Associated Behaviors in Mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal c-Fos antibody Abcam Cat#ab190289, RRID:AB_2737414 CaMKII alpha Monoclonal Antibody (6G9) Thermo Fisher Scientific Cat#MA1-048, RRID:AB_325403 Goat Anti-Rabbit IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor 546 Molecular Probes (Invitrogen) Cat#A-11035, RRID:AB_143051 Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 633 Molecular Probes (Invitrogen) Cat#A-21050, RRID:AB_141431 Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 546 Molecular Probes (Invitrogen) CatA-11003, RRID:AB_141370 Bacterial and Virus Strains AAV9.EF1a.DIO.hChR2(H134R)eYFP.WPRE.hGH Addgene (Karl Deisseroth Lab: Creactivated AAV expression plasmids Unpublished) RRID:Addgene_20298 AAV9.EF1a.DIO.eYFP.WPRE.hGH Addgene (Karl Deisseroth Lab: Double Floxed Inverted ORF Control Unpublished) RRID:Addgene_27056 AAVrg-syn-jGCaMP7s-WPRE Addgene RRID:Addgene_104487 AAV9-Ef1a-DIO C1V1 (t/t)-TS-EYFP Addgene RRID:Addgene_35497 Chemicals SCH 23390 hydrochloride Tocris N/A Cat. No. 0925 (S)-(-)-Sulpiride Tocris N/A Cat. No. 0895 Experimental Models: Organisms/Strains B6.SJL-Slc6a3tm1.1(cre)Bkmn/J Mus musculus Jax RRID:IMSR_JAX:006660 B6;129S-Gt(ROSA) 26Sortm32(CAG-COP4*H134R/EYFP)Hze/J Mus musculus Jax RRID:IMSR_JAX:012569 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus Jax RRID:IMSR_JAX:007909 B6.SJL-Slc6a3tm1.1(cre)Bkmn/J x B6;129S-Gt (ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J Mus musculus University of Calgary animal facility (Origin of breeding pairs: Jax) N/A B6.SJL-Slc6a3tm1.1(cre)Bkmn/J x B6.CgGt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus University of Calgary animal facility (Origin of breeding pairs: Jax) N/A Software and Algorithms OriginPro 9.1 Origin RRID:SCR_014212 MATLAB (R2018a) MATLAB RRID:SCR_001622 GraphPad GraphPad RRID:SCR_000306 pClamp Molecular Devices RRID:SCR_011323 TarHeel CV Software ESA Biosciences N/A High Definition Cyclic Voltammetry Suite University of North Carolina N/A Doric Neuroscience Studio Software Doric Lenses N/A Power analysis tool UBC- Rollin Brant https://www.stat.ubc.ca/ rollin/stats/ ssize/n2.html MATLAB custom code Custom code https://github.com/leomol/FPA (Continued on next page) e1 Cell Reports 31, 107812, June 23, 2020 ..
Software:Article Title: Dopamine Inputs from the Ventral Tegmental Area into the Medial Prefrontal Cortex Modulate Neuropathic Pain-Associated Behaviors in Mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal c-Fos antibody Abcam Cat#ab190289, RRID:AB_2737414 CaMKII alpha Monoclonal Antibody (6G9) Thermo Fisher Scientific Cat#MA1-048, RRID:AB_325403 Goat Anti-Rabbit IgG (H+L) Highly Crossadsorbed Antibody, Alexa Fluor 546 Molecular Probes (Invitrogen) Cat#A-11035, RRID:AB_143051 Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 633 Molecular Probes (Invitrogen) Cat#A-21050, RRID:AB_141431 Goat Anti-Mouse IgG (H+L) Antibody, Alexa Fluor 546 Molecular Probes (Invitrogen) CatA-11003, RRID:AB_141370 Bacterial and Virus Strains AAV9.EF1a.DIO.hChR2(H134R)eYFP.WPRE.hGH Addgene (Karl Deisseroth Lab: Creactivated AAV expression plasmids Unpublished) RRID:Addgene_20298 AAV9.EF1a.DIO.eYFP.WPRE.hGH Addgene (Karl Deisseroth Lab: Double Floxed Inverted ORF Control Unpublished) RRID:Addgene_27056 AAVrg-syn-jGCaMP7s-WPRE Addgene RRID:Addgene_104487 AAV9-Ef1a-DIO C1V1 (t/t)-TS-EYFP Addgene RRID:Addgene_35497 Chemicals SCH 23390 hydrochloride Tocris N/A Cat. No. 0925 (S)-(-)-Sulpiride Tocris N/A Cat. No. 0895 Experimental Models: Organisms/Strains B6.SJL-Slc6a3tm1.1(cre)Bkmn/J Mus musculus Jax RRID:IMSR_JAX:006660 B6;129S-Gt(ROSA) 26Sortm32(CAG-COP4*H134R/EYFP)Hze/J Mus musculus Jax RRID:IMSR_JAX:012569 B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus Jax RRID:IMSR_JAX:007909 B6.SJL-Slc6a3tm1.1(cre)Bkmn/J x B6;129S-Gt (ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J Mus musculus University of Calgary animal facility (Origin of breeding pairs: Jax) N/A B6.SJL-Slc6a3tm1.1(cre)Bkmn/J x B6.CgGt(ROSA)26Sortm9(CAG-tdTomato)Hze/J Mus musculus University of Calgary animal facility (Origin of breeding pairs: Jax) N/A Software and Algorithms OriginPro 9.1 Origin RRID:SCR_014212 MATLAB (R2018a) MATLAB RRID:SCR_001622 GraphPad GraphPad RRID:SCR_000306 pClamp Molecular Devices RRID:SCR_011323 TarHeel CV Software ESA Biosciences N/A High Definition Cyclic Voltammetry Suite University of North Carolina N/A Doric Neuroscience Studio Software Doric Lenses N/A Power analysis tool UBC- Rollin Brant https://www.stat.ubc.ca/ rollin/stats/ ssize/n2.html MATLAB custom code Custom code https://github.com/leomol/FPA (Continued on next page) e1 Cell Reports 31, 107812, June 23, 2020 ..
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